KMID : 0880220070450040344
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Journal of Microbiology 2007 Volume.45 No. 4 p.344 ~ p.349
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Schizosaccharomyces pombe nup97, which Genetically Interacts with mex67, is Essential for Growth and Involved in mRNA Export
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Cho Hyun-Jin
Hwang Duk-Kyung Jung Sun-Im Yoon Jin-Ho
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Abstract
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We have isolated previously three synthetic lethal mutants in Schizosaccharomyces pombe, which genetically interact with mex67, in order to identify the genes involved in mRNA export. A novel nup97 gene was isolated by complementation of the growth defect in one of the synthetic lethal mutants, SLMex3. The nup97 gene contains one intron and encodes an 851 amino-acid protein that is similar to nucleoporins, Npp106p in S. pombe and Nic96p in Saccharomyces cerevisiae. The nup97 gene is essential for vegetative growth, and nup97 null mutant harboring pREP41X-Nup97 showed poly(A)+ RNA export defect when expression of nup97 is repressed in the presence of thiamine. These results suggest that nup97 is involved in mRNA export from the nucleus to cytoplasm
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KEYWORD
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nup97, mex67, synthetic lethality, mRNA export, Schizosaccharomyces pombe
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